Thermo Fisher Scientific has worked rapidly to develop a multiplex real-time RT-PCR diagnostic kit to enable clinical and public health laboratories to quickly diagnose COVID-19 caused by SARS-CoV-2 infection.
TaqPath™ COVID-19 Combo Kit (Applied Biosystems) contains the assays, controls, and master mix required for the real-time PCR detection of RNA from the SARS-CoV-2 virus.
TaqPath™ COVID-19 Combo Kit includes the following components:
- TaqPath COVID-19 RT-PCR Kit, 1,000 reactions
- TaqPath COVID-19 Assay Multiplex – multiplexed assays that contain three primer/probe sets specific to different SARS-CoV-2 genomic regions and primers/probes for bacteriophage MS2
- MS2 Phage Control – a RNA control to verify the efficacy of the sample preparation and the absence of inhibitors in the PCR reaction; to perform the control, add 10 μL of MS2 Phage Control to the samples before extraction of the RNA
- TaqPath COVID-19 Control – RNA control that contains targets specific to the SARS-CoV-2 genomic regions targeted by the assays
The kit can be used by clinical and public health laboratories to quickly evaluate up to 94 patient specimens in under 2 hours. The kit is approved for use with RNA extracted from nasopharyngeal swabs, nasopharyngeal aspirate (nasal aspirate), and bronchoalveolar lavage (BAL) from patients at risk of exposure to the SARS-CoV-2 virus or with signs and symptoms of COVID-19 (see product label for Intended Use Statement and product information).
Features of the newly developed multiplex diagnostic kit:
- Detection using real-time PCR to diagnostic report generation in under 2 hours
- Assays cover all of the SARS-CoV2 genomes of high sequence quality and complete sequence length that are currently available through the GISAID Initiative
- Assays target genomic regions of the SARS-CoV-2 genome that are not present in other coronaviruses (e.g., SARS, MERS, bat SARS-like coronavirus, etc.)
Products from Thermo Fisher Scientific that are related to COVID-19 are regulated and only available to clinical and diagnostic laboratories. Regulations prohibit us from providing products to individual customers. If you are a consumer looking for a COVID-19 test, please contact us.
If you are a looking for homebrew kits, the recommended Master Mixes are:
- TaqPath™ 1-Step RT-qPCR Master Mix which is mentioned also in CDC’s document
- TaqMan™ Fast Virus 1-Step Master Mix which is mentioned also in the following article
- TaqPath™ 1-Step Multiplex Master Mix (No ROX)
- AgPath-ID™ One-Step RT-PCR Reagents
- SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
For more information, please Email to: qPCR@rhenium.co.il