TaqPath COVID-19 Multiplex Diagnostic Solution

In the following newsletters we will go back to basic terms in real time PCR and define them.Here we discuss about the meaning of threshold and where is the best place to set it.

Thermo Fisher Scientific has worked rapidly to develop a multiplex real-time RT-PCR diagnostic kit to enable clinical and public health laboratories to quickly diagnose COVID-19 caused by SARS-CoV-2 infection.

TaqPath™ COVID-19 Combo Kit (Applied Biosystems) contains the assays, controls, and master mix required for the real-time PCR detection of RNA from the SARS-CoV-2 virus.
TaqPath™ COVID-19 Combo Kit includes the following components:

  • TaqPath COVID-19 RT-PCR Kit, 1,000 reactions
    • TaqPath COVID-19 Assay Multiplex – multiplexed assays that contain three primer/probe sets specific to different SARS-CoV-2 genomic regions and primers/probes for bacteriophage MS2
    • MS2 Phage Control – a RNA control to verify the efficacy of the sample preparation and the absence of inhibitors in the PCR reaction; to perform the control, add 10 μL of MS2 Phage Control to the samples before extraction of the RNA
  • TaqPath COVID-19 Control  RNA control that contains targets specific to the SARS-CoV-2 genomic regions targeted by the assays

The kit can be used by clinical and public health laboratories to quickly evaluate up to 94 patient specimens in under 2 hours. The kit is approved for use with RNA extracted from nasopharyngeal swabs, nasopharyngeal aspirate (nasal aspirate), and bronchoalveolar lavage (BAL) from patients at risk of exposure to the SARS-CoV-2 virus or with signs and symptoms of COVID-19 (see product label for Intended Use Statement and product information).
Features of the newly developed multiplex diagnostic kit:

  • Detection using real-time PCR to diagnostic report generation in under 2 hours
  • Assays cover all of the SARS-CoV2 genomes of high sequence quality and complete sequence length that are currently available through the GISAID Initiative
  • Assays target genomic regions of the SARS-CoV-2 genome that are not present in other coronaviruses (e.g., SARS, MERS, bat SARS-like coronavirus, etc.)
​Products from Thermo Fisher Scientific that are related to COVID-19 are regulated and only available to clinical and diagnostic laboratories. Regulations prohibit us from providing products to individual customers. If you are a consumer looking for a COVID-19 test, please contact us.

If you are a looking for homebrew kits, the recommended Master Mixes are:

For more information, please Email to: qPCR@rhenium.co.il