Pharmacogenomics: where pharmacology and genomics meet

A tailor-made medication, based on genetic profile - understand why individuals respond differently to medicines.

Pharmacogenomics (PGx) is the study of genetic variation in relation to drug response and toxicology. This field of science and genomics helps to predict an individual’s response to a drug, allowing to select one medication over the others. It also has the potential to help develop effective, safe medications and doses tailored to an individual’s genetic makeup.

PGx studies focus on testing individuals for multiple variants in drug metabolism enzyme (DME) and transporter genes – including SNPs (single nucleotide polymorphisms), MNPs (multiple nucleotide polymorphisms), and in/dels (insertion/deletions.

Various technologies can be used for pharmacogenomics genotyping. One of them is real-time PCR – a low cost per sample and a fast method suitable for routine testing of samples up to about 120 genetic targets.  

pharmacogenomics real-time PCR genotyping utilizes the Taqman technology:

TaqMan SNP Genotyping Assays use TaqMan 5´ nuclease assay chemistry to amplify and detect specific SNP alleles in purified genomic DNA samples. Each predesigned TaqMan SNP Genotyping Assay includes two allele-specific TaqMan MGB probes containing distinct fluorescent dyes and a PCR primer pair to detect specific SNP targets. These TaqMan probe and primer sets (assays) uniquely align with the genome to provide unmatched specificity for the allele of interest.

 

With thermofisher’s Pharmacogenomic DME Genotyping real time PCR solutions you get a full workflow from detection to analysis including detection of Triallelic SNPs by using Pairs of TaqMan DME Assays. All with predesigned or custom assays and a dedicated data analysis software.

A complete solution gives you:


1. Confidence—established technology
  •   TaqMan® Assays are one of the most trusted real-time PCR technologies available.
2. Flexibility—design your own panel
  • Use a predefined PGx Panel or design your own pharmacogenomic panel by selecting from over 2,700 TaqMan® Drug Metabolism Enzyme (DME) Genotyping Assays targeting the most common DME markers (including CYP2D6, CYP2C9, CYP4F2, and more), 4.5 million predesigned TaqMan® SNP Genotyping Assays, or custom TaqMan® SNP Genotyping Assays.
3. Scalability—choose the format that fits your needs
4. Simplicity—minimize analysis time
  • AlleleTyper™ Software provides an easy and convenient analysis tool for star-  allele nomenclature.
Get a deeper knowledge of all Taqman genomic solutions here.