Digital PCR: To find a needle in a haystack

Higher accuracy, sensitivity and absolute quantification

Digital PCR offers an alternate method to conventional qPCR – for absolute quantification and detection with no need to rely on references or standards.

Digital PCR provides increased precision by partitioning a sample into thousands of PCR reactions in parallel. Using TaqMan chemistry with dye-labeled probes, each well is individually analyzed, to detect the presence or absence of an endpoint signal and quantify the number of positive wells.

Case study of rare fusion transcript detection is summarized in this application note.